Part:BBa_J23075:Design

[RA2 Promoter][RBS][trbCf][TT]

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 568
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal PstI site found at 568
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 568
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 568
  Illegal NgoMIV site found at 648
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

pSB1A2-J23038 has a really short sequence between XbaI and SpeI so okay to PCR because miniprep was messy.

Source

[RA2 Promoter][RBS][trbCf][TT] Digest PCR product of pSB1A2-J23038 with G00101 and ca641R (BSAI/SpeI, Neb2, ~1600 ) Paste into pSB1AK3-J23028 (XbaI/BsaI, Neb2, Large 5229) Product is pSB1AK3-J23075.